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lentivirus particles expressing negative control with irrelevant shrna  (Shanghai GenePharma)

 
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    Structured Review

    Shanghai GenePharma lentivirus particles expressing negative control with irrelevant shrna
    Lentivirus Particles Expressing Negative Control With Irrelevant Shrna, supplied by Shanghai GenePharma, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/irrelevant+control+shrnas/shrna+lentivirus/10__1096_slash_fj__202101555rr-38-6-20
    Average 90 stars, based on 1 article reviews
    lentivirus particles expressing negative control with irrelevant shrna - by Bioz Stars, 2026-10
    90/100 stars

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    shRNA:

    Article Title: MicroRNA-593-3p regulates insulin-promoted glucose consumption by targeting Slc38a1 and CLIP3
    Article Snippet: 89 Chemically synthesized miRNA mimics and miRNA inhibitors were purchased from RiboBio 90 (Guangzhou, China). .. The short hairpin RNA (shRNA) and irrelevant control shRNAs were 91 purchased from Genepharma (Shanghai, China) and target sequences are listed in 92 Supplementary Table 1. ..

    Control:

    Article Title: MicroRNA-593-3p regulates insulin-promoted glucose consumption by targeting Slc38a1 and CLIP3
    Article Snippet: 89 Chemically synthesized miRNA mimics and miRNA inhibitors were purchased from RiboBio 90 (Guangzhou, China). .. The short hairpin RNA (shRNA) and irrelevant control shRNAs were 91 purchased from Genepharma (Shanghai, China) and target sequences are listed in 92 Supplementary Table 1. ..



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    Fig. 6 PRDX-1 knockdown sensitises IR resistant NG2 positive cells in vivo. a Schematic of an intracerebral tumour-bearing mouse implanted with 125I seeds. A gradient IR dose (fading line) was delivered to the tumour bed. B brain, S guide-screw, T tumour, n number of animals (adapted from [50]). b Tumour sizes in vivo before IR treatment (top panel) and after treatment with 125I (bottom panel). c Histological staining at 19 weeks with H&E and anti- NG2 antibodies. Note the vascular lakes in the U251-NG2 control <t>shRNA</t> and numerous mitotic figures (arrowheads) in U251-NG2/PRDX-1 shRNA tumours, giant multinucleated cells (arrowheads). Scale bars in all (100 lm; magnification 4009), except rows stained for NG2 (200 lm; magnification 2009). d Densitometric quantified, Fold PRDX-1 change in tumour compared to normal brain tissue of mice receiving no treatment or ionizing radiation
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    Fig. 6 PRDX-1 knockdown sensitises IR resistant NG2 positive cells in vivo. a Schematic of an intracerebral tumour-bearing mouse implanted with 125I seeds. A gradient IR dose (fading line) was delivered to the tumour bed. B brain, S guide-screw, T tumour, n number of animals (adapted from [50]). b Tumour sizes in vivo before IR treatment (top panel) and after treatment with 125I (bottom panel). c Histological staining at 19 weeks with H&E and anti- NG2 antibodies. Note the vascular lakes in the U251-NG2 control shRNA and numerous mitotic figures (arrowheads) in U251-NG2/PRDX-1 shRNA tumours, giant multinucleated cells (arrowheads). Scale bars in all (100 lm; magnification 4009), except rows stained for NG2 (200 lm; magnification 2009). d Densitometric quantified, Fold PRDX-1 change in tumour compared to normal brain tissue of mice receiving no treatment or ionizing radiation

    Journal: Acta neuropathologica

    Article Title: Expression of the progenitor marker NG2/CSPG4 predicts poor survival and resistance to ionising radiation in glioblastoma.

    doi: 10.1007/s00401-011-0867-2

    Figure Lengend Snippet: Fig. 6 PRDX-1 knockdown sensitises IR resistant NG2 positive cells in vivo. a Schematic of an intracerebral tumour-bearing mouse implanted with 125I seeds. A gradient IR dose (fading line) was delivered to the tumour bed. B brain, S guide-screw, T tumour, n number of animals (adapted from [50]). b Tumour sizes in vivo before IR treatment (top panel) and after treatment with 125I (bottom panel). c Histological staining at 19 weeks with H&E and anti- NG2 antibodies. Note the vascular lakes in the U251-NG2 control shRNA and numerous mitotic figures (arrowheads) in U251-NG2/PRDX-1 shRNA tumours, giant multinucleated cells (arrowheads). Scale bars in all (100 lm; magnification 4009), except rows stained for NG2 (200 lm; magnification 2009). d Densitometric quantified, Fold PRDX-1 change in tumour compared to normal brain tissue of mice receiving no treatment or ionizing radiation

    Article Snippet: Retroviral PRDX-1 and irrelevant GFP control shRNA constructs were purchased from Origene (Rockville, MD), and used to generate the stable U251-NG2 shPRDX-1 and U251-NG2-shCTRL cell lines as well control U251 shPRDX-1 and U251 shCTRL cell lines.

    Techniques: Knockdown, In Vivo, Staining, Control, shRNA